Free and open source

Nuclei Segmentation using Deep Learning for individual cell analysis (DeepCell).

This plugin computes for each image element (pixel/voxel) the eigenvalues of the Hessian, which can be used for example to discriminate locally between plate-like, line-like, and blob-like image structures

3D spot detection using the Determinant of Hessian (DoH) and the detection of 3D minima.

Spot detection in 3D images by Wavelet Adaptive Threshold in Icy.

This workflow detects spots in a 2D image by filtering the image by Laplacian of Gaussian (user defined radius), thresholding (user defined threshold) and finding local intensity maxima in mask distance map (Dmap).