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Description

Image-processing algorithms developed at the MOSAIC Group for fluorescence microscopy. Tools included:

  • 2D/3D single-particle tracking tool which can be used to track bright spots in 2D/3D movies over time.
  • Optimal filament segmentation of 2D images. 
  • Curvature filters for image filtering, denoising, and restoration. 
  • Image naturalization for image enhancement based on gradient statistics of natural-scence images. 
  • Tool for automatically send and distribute jobs on clusters and get back the results.
  • Multi-region image segmentation of 2D and 3D images without needing to know the number of regions beforehand. 
  • Squassh for globally optimal segmentation of piecewise constant regions in 2D and 3D images and for object-based co-localization analysis. 
  • Tool for inferring spatial interactions between patterns of objects in images or between coordinates read from a file.
  • Tool for robust, histogram-based background subtraction well suited to correct for inhomogeneous illumination artifacts.
  • A tool to estimate the Point-Spread Function of the microscopy out of 2D fluorescence images.
  • A tool to measure the 3D Point-Spread Function of a confocal microscope from an image stack.
  • Addition of synthetic Poisson-distributed noise to an image in order to simulate shot noise of various signal-to-noise ratios. 
  • Convolution of an image with a Bessel function in order to simulate imaging with a microscope. 
  • A utility to detect bright spots in images and estimate their center. 
  • A utility to create manual segmentations to be used as ground truth to test and benchmark automatic segmentation algorithms.
  • A tool for replacing one color in an image with another color.
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Description

arivis Vision4D is a modular software for working with multi-channel 2D, 3D and 4D images of almost unlimited size independent of available RAM. Many imaging systems, such as high speed confocal, Light Sheet/ SPIM and 2 Photon systems, can produce a huge amount of multi-channel data, which arivis Vision4D handles without constraints. Terabyte ready arivis Vision4D main functionality: Easy import of most image formats from microsopes as well as biological formats High performance interactive 3D / 4D rendering on standard PCs and laptops with 3D Graphics Support Intuitive tools for stitching and alignment to create large multi-dimensional image stacks Immediate 2D, 3D and 4D visualization, annotation and analysis regardless of image size Creation, import, and export of 4D Iso-surfaces Powerful Analysis Pipeline for 3D /4D image analysis (cell segmentation, tracking, annotation, quantitative measurement and statistics, etc) Semi-automatic/manual segmentation and tracking with interactive Track Editor Easy design and export of 3D / 4D High Resolution Movies Seamless integration of custom workflows via Matlab API and Python scripting Data sharing for collaboration A user friendly software, easy to learn and use for any life scientist

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Description

MosaicIA is a tool to analyze the spatial distribution of objects in images. It estimates from an observed particle or object distribution what hypothetical interaction between the objects is most likely to have created this distribution.

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Cytomine is a rich internet application using modern web and distributed technologies (Grails, HTML/CSS/Javascript, Docker), databases (spatial SQL and NoSQL), and machine learning (tree-based approaches with random subwindows) to foster active and distributed collaboration and ease large-scale image exploitation.

It provides remote and collaborative principles, rely on data models that allow to easily organize and semantically annotate imaging datasets in a standardized way (using user-defined ontologies associated to regions of interest), efficiently support high-resolution multi-gigapixel images (incl. major digital scanner image formats), and provide mechanisms to readily proofread and share image quantifications produced by any image recognition algorithms.

By emphasizing collaborative principles, the aim of Cytomine is to accelerate scientific progress and to significantly promote image data accessibility and reusability. Cytomine allows to break common practices in this domain where imaging datasets, quantification results, and associated knowledge are still often stored and analyzed within the restricted circle of a specific laboratory.

This software is e.g. being used by life scientists in to help them better evaluate drug treatments or understand biological processes directly from whole-slide tissue images (digital histology), by pathologists to share and ease their diagnosis, and by teachers and students for pathology training purposes. It is also used in various microscopy applications.

Cytomine can be used as a stand-alone application (e.g. on a laptop) or on larger servers for collaborative works.

Cytomine implements object classification, image segmentation, content-based image retrieval, object counting, and interest point detection algorithms using machine learning.

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Description

## Short Summary Quote from the plugin page: >LineageTracker offers an ImageJ based framework which is easily extendible and has the capability to track cell lineages while being specifically designed to handle large cell displacements between frames. The methods are designed for fluorescent cells and have been used to analyse Schizosaccharomyces pombe, C2C12 mouse stem cells or migrating RPE cells. This tool also allows flexible cell segmentation and extendable in all aspects. The webpage is detailed with usage from ImageJ macro. Rather than being simply a component, the plugin is indeed a framework with set of components. ## Misc info A tip from the plugin author in ImageJ mailing list (08.Sep.2015): > We have an additional script to export only a selected range of frames. I can send you that if you think LineageTracker is something for you. To be on the safe side I would try it with an older version of ImageJ. We have experienced some problems, mostly related to Java. Java 8 seems to fix most of it. ## References 2630: Application example. 2631: Plugin Paper.

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