3D

Yet another pixel classifier Yapic is a deep learning tool to :

train your own filter to enhance the structure of your choice 

train multiple filter at once 

it is based on the u-net convolutional filter . 

To train it : annotation can come from example from Ilastik software , tif labelled files can be transferred to yapic. 

Training takes about hours to days , prediction takes seconds once trained .

It can be ran from command line .

note that only 10 to 20 images with sparse labeling are required for efficient training 

LOBSTER (Little Objects Segmentation and Tracking Environment), an environment designed to help scientists design and customize image analysis workflows to accurately characterize biological objects from a broad range of fluorescence microscopy images, including large images, i.e. terabytes of data, exceeding workstation main memory.

• 75 workflows available 
• no programming, with GUI
• matlab based 

This is the ImageJ/Fiji plugin for StarDist, a cell/nuclei detection method for microscopy images with star-convex shape priors ( typically for Dapi like staining of nuclei). The plugin can be used to apply already trained models to new images.

Summary

Deep learning-based segmentation of cells, both fluorescence, and bright-field images ("a generalist algorithm for cellular segmentation"). The tool can be used either online or local or via notebooks (e.g. ZeroCostDL4Mic).

How to use it

cellpose can be used online via ready-to-use Jyupyter notebooks with very good documentation. These notebooks are listed here.

Local Installation

The general local installation procedure can be found here.

... Installing to Silicon Mac (M1 processor) needs some tricks, and as of October 2021, the following sequence of commands works. numba should be conda-installed before pip-installing the cellpose.

conda create --name cellpose python=3.8
conda activate cellpose
conda install numba
git clone https://github.com/MouseLand/cellpose.git
cd cellpose
pip install -e .

The Morphonet Python API provide an easy interface to interact directly with the MorphoNet server. Very useful to upload, download your dataset and superimpose on it any quantitative and quantitative informations.