Microtubule end tracking in Drosophila Oocyte

Type
Author
Graeme Ball
Execution Platform
Supported image dimension
Interaction Level
License/Openness
Description
Microtubule end tracking in live cell fluorescent images of Drosophila oocyte involves overcoming the following challenges, which can be tackled by a series of preprocessing steps and tracking described in Parton et al (2011) * **illumination flicker & photobleaching**: suppress by normalising intensities, e.g. using Image->Adjust->Bleach Correction in Fiji/ImageJ * **uneven illumination**: Fourier bandpass filtering (e.g. Process->FFT->Bandpass Filter) preserves features within a selected size range * **high background / poor contrast**: foreground filter, e.g. Temporal Median filter * **tracking**: e.g. TrackMate in Fiji/ImageJ (segmentation using DoG detector)
has function
Entry Curator