Contents

Matlab implementation (2014) of Local Binary Pattern. Used for texture image analysis with insensitivity to local average value. Good explanation here: http://www.ee.oulu.fi/research/imag/mvg/files/pdf/ICCV2009_tutorial.pdf

This package contains some MatLab tools for multi-scale image processing. Briefly, the tools include: - Recursive multi-scale image decompositions (pyramids), including Laplacian pyramids, QMFs, Wavelets, and steerable pyramids. These operate on 1D or 2D signals of arbitrary dimension. Data structures are compatible with the MatLab wavelet toolbox. - Fast 2D convolution routines, with subsampling and boundary-handling. - Fast point-operations, histograms, histogram-matching. - Fast synthetic image generation: sine gratings, zone plates, fractals, etc.

A workflow in Python to measure muscule fibers corresponding to the method used in Keefe, A.C. et al. Muscle stem cells contribute to myofibres in sedentary adult mice. Nat. Commun. 6:7087 doi: 10.1038/ncomms8087 (2015).

Example image:

muscleQNT/15536_2032_0.tif ...

It is a trainable interest point (anatomical landmarks) detection algorithm. It requires images and interest point coordinates. It can run independantly (using csv files to describe coordinates) or it can be executed using Cytomine.

Typical application: Morphometric studies (e.g. in zebrafish/drosphila development)

Neurolucida is a powerful tool for creating and analyzing realistic, meaningful, and quantifiable neuron reconstructions from microscope images. Perform detailed morphometric analysis of neurons, such as quantifying 1) the number of dendrites, axons, nodes, synapses, and spines, 2) the length, width, and volume of dendrites and axons, 3) the area and volume of the soma, and 4) the complexity and extension of neurons. See 10.3389/fnins.2012.00049

SIMcheck is an ImageJ plugin suite for assessing the quality and reliability of Structured Illumination Microscopy (SIM) data. The quality of the raw data, the quality of the reconstruction and the calibration of the microscope can be tested. 

This is the "prediction step" of the Pyxit segmentation model builder. It is a learnable segmentation algorithm based on ground-truth images and segmentation mask. It learns a multiple output pixel classification algorithm. It downloads from Cytomine-Core annotation images+alphamasks from project(s), build a segmentation (pixel classifier) model which is saved locally. Typical application: tumor detection in tissues in histology slides. 

This is a learnable segmentation algorithm based on ground-truth images and segmentation mask. It learns a multiple output pixel classification algorithm. It downloads from Cytomine-Core annotation images+alphamasks from project(s), build a segmentation (pixel classifier) model which is saved locally. Typical application: tumor detection in tissues in histology slides.

Nuget package for conversion between color spaces and calculation of color differences. Color spaces available: -CMY -CMYK -HSL -HSB -HSV -CIE L*a*b* -Hunter LAB -L*C*h* -L*u*v* -RGB -XYZ -YXY Color differences available: -CIE76 -CMC l:c -CIE94 -CIE2000. Online example at http://colormine.org/color-converter

This module is for applying classification models on objects. It downloads from Cytomine-Core annotation images and coordinate of annotated objects from project(s) and build a annotation classification model which is saved locally. It downloads from Cytomine-Core annotations images from an image (e.g. detected by an object finder), apply a classification model (previously saved locally), and uploads to Cytomine-Core annotation terms (in a userjob layer).

This module is for learning classification models from ground-truth data (supervised learning). It downloads from Cytomine-Core annotation images and coordinate of annotated objects from project(s) and build a annotation classification model which is saved locally.  

It is used by Cytomine DataMining applications: classification_validation, classification_model_builder, classification_prediction, segmentation_model_builder and segmentation_prediction. But it can be run without Cytomine on local data (using dir_ls and dir_ts arguments).

SLDC is an open-source Python workflow. SLDC stands for Segment Locate Dispatch Classify. This framework aims at facilitating the development of algorithms for detecting objects in multi-gigapixel images. Particularly, it provides algorithm developers with a structure to define problem-dependent components of their processing workflow (i.e. segmentation and classification) in a concise way. Every other concern such as parallelization and large image handling are encapsulated by the framework.

Automatic registration in 2D or 3D based on detection or binary mask. Takes images with detections already done on it.

This plugin allows to compute a similarity (translation/rotation/scaling and flipping) transform from pair of points. It is updating the transformed image interactively such that the user get immediate feedback. The transformation is saved and can be applied to any other stack/image. Non rigid deformation can also be applied in 2D or 3D.

3D/3D,2D/3D or 3D /2D can be handled .

3D ROI are enabled, and can be checked with the 3D vtk view (size of ROI can be changed using the ROI stroke width).

Some prealignment by rotating in 3D the volume is possible.

In this chapter you will learn to track cell movements within the monolayer epithelium of a Drosophila embryo.The segmentation of the cells is performed by an ImageJ macro on microscope time lapses (movies) of the embryo presented in the maximum intensity projection (step1). Another macro can optionally be used to discard weak cell–cell junctions that are likely to be segmentation errors(step2). Thet racking is then performed in Matlab from this binary stack (step3).

Purpose:

Many tools for BioImage Analysis are already available but information about these tools is non-uniform and often focuses on technicalities about the methods implemented rather than the problems the tools can actually solve. Since bioimage analysts focus on applied problems, this information is often inadequate.

The jicbioimage Python package makes it easy to explore microscopy data in a programmatic fashion (python).

Exploring images via coding means that the exploratory work becomes recorded and reproducible.

Furthermore, it makes it easier to convert the exploratory work into (semi) automated analysis work flows.

Features:

• Built in functionality for working with microscopy data
• Automatic generation of audit trails
• Python integration Works with Python 2.7, 3.3 and 3.4